8J8P

Structure of the four-component Paf1 complex from Saccharomyces eubayanus

8J8P の概要

• エントリーDOI: 10.2210/pdb8j8p/pdb
• 分子名称: CTR9-like protein, CDC73-like protein, PAF1-like protein, ... (5 entities in total)
• 機能のキーワード: transcription elongation factor, the polymerase associated factor 1 (paf1) complex, transcription
• 由来する生物種: Saccharomyces eubayanus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 135378.64

構造登録者

Wang, Z.,Qin, Y.,Zhou, Y.,Cao, Y. (登録日: 2023-05-02, 公開日: 2023-05-24, 最終更新日: 2024-05-29)

主引用文献

Qin, Y.,Zhou, Y.,Cao, Y.,Ren, Y.,Deng, P.,Jiang, J.,Wang, Z.
Structural Basis of the Transcriptional Elongation Factor Paf1 Core Complex from Saccharomyces eubayanus .
Int J Mol Sci, 24:-, 2023

PubMed Abstract: The multicomponent polymerase associated factor 1 (Paf1) complex (PAF1C) is an important transcription elongation factor that upregulates RNA polymerase II-mediated genome-wide transcription. PAF1C can regulate transcription through direct association with the polymerase or by impacting the chromatin structure epigenetically. In recent years, significant progress has been made in understanding the molecular mechanisms of PAF1C. However, high-resolution structures that can clarify the interaction details among the components of the complex are still needed. In this study, we evaluated the structural core of the yeast PAF1C containing the four components Ctr9, Paf1, Cdc73 and Rtf1 at high resolution. We observed the interaction details among these components. In particular, we identified a new binding surface of Rtf1 on PAF1C and found that the C-terminal sequence of Rtf1 dramatically changed during evolution, which may account for its different binding affinities to PAF1C among species. Our work presents a precise model of PAF1C, which will facilitate our understanding of the molecular mechanism and the in vivo function of the yeast PAF1C.

PubMed: 37240075
DOI: 10.3390/ijms24108730

実験手法

X-RAY DIFFRACTION (2.7 Å)