8J4U
Structure of HerA-Sir2 complex from Escherichia coli Nezha system
This is a non-PDB format compatible entry.
Summary for 8J4U
| Entry DOI | 10.2210/pdb8j4u/pdb |
| EMDB information | 35978 |
| Descriptor | SIR2-like domain-containing protein, Nucleoside triphosphate hydrolase, [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL[HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE, ... (5 entities in total) |
| Functional Keywords | defense system hera sir2, immune system |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 18 |
| Total formula weight | 981756.25 |
| Authors | |
| Primary citation | Tang, D.,Chen, Y.,Chen, H.,Jia, T.,Chen, Q.,Yu, Y. Multiple enzymatic activities of a Sir2-HerA system cooperate for anti-phage defense. Mol.Cell, 83:4600-4613.e6, 2023 Cited by PubMed Abstract: In response to the persistent exposure to phage infection, bacteria have evolved diverse antiviral defense mechanisms. In this study, we report a bacterial two-component defense system consisting of a Sir2 NADase and a HerA helicase. Cryo-electron microscopy reveals that Sir2 and HerA assemble into a ∼1 MDa supramolecular octadecamer. Unexpectedly, this complex exhibits various enzymatic activities, including ATPase, NADase, helicase, and nuclease, which work together in a sophisticated manner to fulfill the antiphage function. Therefore, we name this defense system "Nezha" after a divine warrior in Chinese mythology who employs multiple weapons to defeat enemies. Our findings demonstrate that Nezha could sense phage infections, self-activate to arrest cell growth, eliminate phage genomes, and subsequently deactivate to allow for cell recovery. Collectively, Nezha represents a paradigm of sophisticated and multifaceted strategies bacteria use to defend against viral infections. PubMed: 38096825DOI: 10.1016/j.molcel.2023.11.010 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.97 Å) |
Structure validation
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