8J4C
YeeE(TsuA)-YeeD(TsuB) complex for thiosulfate uptake
Summary for 8J4C
| Entry DOI | 10.2210/pdb8j4c/pdb |
| Descriptor | Spirochaeta thermophila YeeE(TsuA)-YeeD(TsuB),UPF0033 domain-containing protein, SirA-like domain-containing protein (chimera), THIOSULFATE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (3 entities in total) |
| Functional Keywords | thiosulfate, bacterial sulfur assimilation, membrane protein, transport protein |
| Biological source | Spirochaeta thermophila DSM 6578 More |
| Total number of polymer chains | 2 |
| Total formula weight | 106666.89 |
| Authors | Ikei, M.,Miyazaki, R.,Monden, K.,Naito, Y.,Takeuchi, A.,Takahashi, Y.S.,Tanaka, Y.,Ichikawa, M.,Tsukazaki, T. (deposition date: 2023-04-19, release date: 2024-03-27, Last modification date: 2024-10-09) |
| Primary citation | Ikei, M.,Miyazaki, R.,Monden, K.,Naito, Y.,Takeuchi, A.,Takahashi, Y.S.,Tanaka, Y.,Murata, K.,Mori, T.,Ichikawa, M.,Tsukazaki, T. YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition. Plos Biol., 22:e3002601-e3002601, 2024 Cited by PubMed Abstract: Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of S. thermophila YeeE-YeeD fusion proteins at 3.34-Å and 2.60-Å resolutions revealed their interactions. The association was evaluated by a binding assay using purified S. thermophila YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed. PubMed: 38656967DOI: 10.1371/journal.pbio.3002601 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.34 Å) |
Structure validation
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