8J48

Crystal structure of OY phytoplasma SAP05 in complex with AtGATA18

8J48 の概要

• エントリーDOI: 10.2210/pdb8j48/pdb
• 分子名称: GATA transcription factor 18, Sequence-variable mosaic (SVM) signal sequence domain-containing protein, ZINC ION, ... (4 entities in total)
• 機能のキーワード: ubiquitin-independent protein degradation, plant protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 36035.08

構造登録者

Dong, C.,Yan, X.,Yuan, X. (登録日: 2023-04-19, 公開日: 2024-02-28, 最終更新日: 2024-05-08)

主引用文献

Yan, X.,Yuan, X.,Lv, J.,Zhang, B.,Huang, Y.,Li, Q.,Ma, J.,Li, Y.,Wang, X.,Li, Y.,Yu, Y.,Liu, Q.,Liu, T.,Mi, W.,Dong, C.
Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors.
Nat Commun, 15:1170-1170, 2024

PubMed Abstract: SAP05, a secreted effector by the obligate parasitic bacteria phytoplasma, bridges host SPL and GATA transcription factors (TFs) to the 26 S proteasome subunit RPN10 for ubiquitination-independent degradation. Here, we report the crystal structures of SAP05 in complex with SPL5, GATA18 and RPN10, which provide detailed insights into the protein-protein interactions involving SAP05. SAP05 employs two opposing lobes with an acidic path and a hydrophobic path to contact TFs and RPN10, respectively. Our crystal structures, in conjunction with mutagenesis and degradation assays, reveal that SAP05 targets plant GATAs but not animal GATAs dependent on their direct salt-bridged electrostatic interactions. Additionally, SAP05 hijacks plant RPN10 but not animal RPN10 due to structural steric hindrance and the key hydrophobic interactions. This study provides valuable molecular-level information into the modulation of host proteins to prevent insect-borne diseases.

PubMed: 38326322
DOI: 10.1038/s41467-024-45521-7

実験手法

X-RAY DIFFRACTION (1.94 Å)