8J2M
The truncated rice Na+/H+ antiporter SOS1 (1-976) in a constitutively active state
Summary for 8J2M
| Entry DOI | 10.2210/pdb8j2m/pdb |
| Related | 8IWO |
| EMDB information | 35775 35950 |
| Descriptor | Na+/H+ antiporter, (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl tetradecanoate (2 entities in total) |
| Functional Keywords | na+/h+ antiporter, cation:proton antiporter family 1, membrane protein |
| Biological source | Oryza sativa Japonica Group (Japanese rice) |
| Total number of polymer chains | 2 |
| Total formula weight | 234392.80 |
| Authors | Zhang, X.Y.,Tang, L.H.,Zhang, C.R.,Nie, J.W.,Chen, Y.H. (deposition date: 2023-04-14, release date: 2023-11-22, Last modification date: 2024-11-13) |
| Primary citation | Zhang, X.Y.,Tang, L.H.,Nie, J.W.,Zhang, C.R.,Han, X.,Li, Q.Y.,Qin, L.,Wang, M.H.,Huang, X.,Yu, F.,Su, M.,Wang, Y.,Xu, R.M.,Guo, Y.,Xie, Q.,Chen, Y.H. Structure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na + /H + antiporter. Nat.Plants, 9:1924-1936, 2023 Cited by PubMed Abstract: Salinity is one of the most severe abiotic stresses that adversely affect plant growth and agricultural productivity. The plant Na/H antiporter Salt Overly Sensitive 1 (SOS1) located in the plasma membrane extrudes excess Na out of cells in response to salt stress and confers salt tolerance. However, the molecular mechanism underlying SOS1 activation remains largely elusive. Here we elucidate two cryo-electron microscopy structures of rice (Oryza sativa) SOS1, a full-length protein in an auto-inhibited state and a truncated version in an active state. The SOS1 forms a dimeric architecture, with an NhaA-folded transmembrane domain portion in the membrane and an elongated cytosolic portion of multiple regulatory domains in the cytoplasm. The structural comparison shows that SOS1 adopts an elevator transport mechanism accompanied by a conformational transition of the highly conserved Pro in the unwound transmembrane helix 5 (TM), switching from an occluded conformation in the auto-inhibited state to a conducting conformation in the active state. These findings allow us to propose an inhibition-release mechanism for SOS1 activation and elucidate how SOS1 controls Na homeostasis in response to salt stress. PubMed: 37884653DOI: 10.1038/s41477-023-01551-5 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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