8IXK

bottom segment of the bacteriophage M13 mini variant

8IXK の概要

• エントリーDOI: 10.2210/pdb8ixk/pdb
• EMDBエントリー: 35794
• 分子名称: Attachment protein G3P, Capsid protein G8P, Head virion protein G6P (3 entities in total)
• 機能のキーワード: viral coat protein, m13, viral protein
• 由来する生物種: Inovirus M13; 詳細
• タンパク質・核酸の鎖数: 25
• 化学式量合計: 389154.29

構造登録者

Xiang, Y.,Jia, Q. (登録日: 2023-04-01, 公開日: 2023-09-13, 最終更新日: 2023-09-20)

主引用文献

Jia, Q.,Xiang, Y.
Cryo-EM structure of a bacteriophage M13 mini variant.
Nat Commun, 14:5421-5421, 2023

PubMed Abstract: Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.

PubMed: 37669979
DOI: 10.1038/s41467-023-41151-7

実験手法

ELECTRON MICROSCOPY (3.3 Å)