8IVJ

Candida boidinii Formate Dehydrogenase V120T Mutant

8IVJ の概要

• エントリーDOI: 10.2210/pdb8ivj/pdb
• 分子名称: Formate dehydrogenase (2 entities in total)
• 機能のキーワード: candida boidinii, formate dehydrogenase, fdh, oxidoreductase
• 由来する生物種: [Candida] boidinii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 161467.76

構造登録者

Gul, M.,Yuksel, B.,Bulut, H.,DeMirci, H. (登録日: 2023-03-28, 公開日: 2023-05-10, 最終更新日: 2023-11-08)

主引用文献

Gul, M.,Yuksel, B.,Bulut, H.,DeMirci, H.
Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography.
Acta Crystallogr D Struct Biol, 79:1010-1017, 2023

PubMed Abstract: Candida boidinii NAD-dependent formate dehydrogenase (CbFDH) has gained significant attention for its potential application in the production of biofuels and various industrial chemicals from inorganic carbon dioxide. The present study reports the atomic X-ray crystal structures of wild-type CbFDH at cryogenic and ambient temperatures, as well as that of the Val120Thr mutant at cryogenic temperature, determined at the Turkish Light Source `Turkish DeLight'. The structures reveal new hydrogen bonds between Thr120 and water molecules in the active site of the mutant CbFDH, suggesting increased stability of the active site and more efficient electron transfer during the reaction. Further experimental data is needed to test these hypotheses. Collectively, these findings provide invaluable insights into future protein-engineering efforts that could potentially enhance the efficiency and effectiveness of CbFDH.

PubMed: 37860962
DOI: 10.1107/S2059798323008070

実験手法

X-RAY DIFFRACTION (1.9 Å)