8IR4

Crystal structure of the SLF1 BRCT domain in complex with a Rad18 peptide containing pS442

Summary for 8IR4

• Entry DOI: 10.2210/pdb8ir4/pdb
• Related: 8IR2
• Descriptor: SMC5-SMC6 complex localization factor protein 1, SER-ASP-SER-CYS-ASN-SER-SEP-SER-SER-ASP-ILE-ILE-ARG-ASP-LEU-LEU-GLU, ISOPROPYL ALCOHOL, ... (6 entities in total)
• Functional Keywords: slf1, brct, gene regulation
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 52248.67

Authors

Xiang, S.,Huang, W.,Qiu, F. (deposition date: 2023-03-17, release date: 2023-11-15, Last modification date: 2024-10-16)

Primary citation

Huang, W.,Qiu, F.,Zheng, L.,Shi, M.,Shen, M.,Zhao, X.,Xiang, S.
Structural insights into Rad18 targeting by the SLF1 BRCT domains.
J.Biol.Chem., 299:105288-105288, 2023

PubMed Abstract: Rad18 interacts with the SMC5/6 localization factor 1 (SLF1) to recruit the SMC5/6 complex to DNA damage sites for repair. The mechanism of the specific Rad18 recognition by SLF1 is unclear. Here, we present the crystal structure of the tandem BRCT repeat (tBRCT) in SLF1 (SLF1) bound with the interacting Rad18 peptide. Our structure and biochemical studies demonstrate that SLF1 interacts with two phosphoserines and adjacent residues in Rad18 for high-affinity and specificity Rad18 recognition. We found that SLF1 utilizes mechanisms common among tBRCTs as well as unique ones for Rad18 binding, the latter include interactions with an α-helical structure in Rad18 that has not been observed in other tBRCT-bound ligand proteins. Our work provides structural insights into Rad18 targeting by SLF1 and expands the understanding of BRCT-mediated complex assembly.

PubMed: 37748650
DOI: 10.1016/j.jbc.2023.105288

Experimental method

X-RAY DIFFRACTION (1.62 Å)