8IR2

Crystal structure of the SLF1 BRCT domain in complex with a Rad18 peptide containing pS442 and pS444

8IR2 の概要

• エントリーDOI: 10.2210/pdb8ir2/pdb
• 分子名称: SMC5-SMC6 complex localization factor protein 1, SER-ASP-SER-CYS-ASN-SER-SEP-SER-SEP-ASP-ILE-ILE-ARG-ASP-LEU-LEU-GLU, ISOPROPYL ALCOHOL, ... (6 entities in total)
• 機能のキーワード: slf1, brct, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52419.77

構造登録者

Xiang, S.,Huang, W.,Qiu, F. (登録日: 2023-03-17, 公開日: 2023-11-15, 最終更新日: 2024-11-06)

主引用文献

Huang, W.,Qiu, F.,Zheng, L.,Shi, M.,Shen, M.,Zhao, X.,Xiang, S.
Structural insights into Rad18 targeting by the SLF1 BRCT domains.
J.Biol.Chem., 299:105288-105288, 2023

PubMed Abstract: Rad18 interacts with the SMC5/6 localization factor 1 (SLF1) to recruit the SMC5/6 complex to DNA damage sites for repair. The mechanism of the specific Rad18 recognition by SLF1 is unclear. Here, we present the crystal structure of the tandem BRCT repeat (tBRCT) in SLF1 (SLF1) bound with the interacting Rad18 peptide. Our structure and biochemical studies demonstrate that SLF1 interacts with two phosphoserines and adjacent residues in Rad18 for high-affinity and specificity Rad18 recognition. We found that SLF1 utilizes mechanisms common among tBRCTs as well as unique ones for Rad18 binding, the latter include interactions with an α-helical structure in Rad18 that has not been observed in other tBRCT-bound ligand proteins. Our work provides structural insights into Rad18 targeting by SLF1 and expands the understanding of BRCT-mediated complex assembly.

PubMed: 37748650
DOI: 10.1016/j.jbc.2023.105288

実験手法

X-RAY DIFFRACTION (1.75 Å)