8INP

A reversible glycosyltransferase of tectorigenin - Bc7OUGT

8INP の概要

• エントリーDOI: 10.2210/pdb8inp/pdb
• 分子名称: Bc7OUGT, URIDINE-5'-DIPHOSPHATE, beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: glycosyltransferase, ugt, gt-b fold structure, rossmann-like domain., transferase
• 由来する生物種: Iris domestica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52275.33

構造登録者

Zhang, Z.Y.,Lu, L.,Guan, Z.F.,Cheng, W.J. (登録日: 2023-03-10, 公開日: 2023-08-23, 最終更新日: 2023-11-29)

主引用文献

Cheng, W.,Fang, X.,Guan, Z.,Yao, Y.,Xu, Z.,Bi, Y.,Ren, K.,Li, J.,Chen, F.,Chen, X.,Ma, W.,Chu, Z.,Deng, Z.,Zhang, Z.,Lu, L.
Functional characterization and structural basis of a reversible glycosyltransferase involves in plant chemical defence.
Plant Biotechnol J, 21:2611-2624, 2023

PubMed Abstract: Plants experience numerous biotic stresses throughout their lifespan, such as pathogens and pests, which can substantially affect crop production. In response, plants have evolved various metabolites that help them withstand these stresses. Here, we show that two specialized metabolites in the herbaceous perennial Belamcanda chinensis, tectorigenin and its glycoside tectoridin, have diverse defensive effects against phytopathogenic microorganisms and antifeeding effects against insect pest. We further functionally characterized a 7-O-uridine diphosphate glycosyltransferase Bc7OUGT, which catalyses a novel reversible glycosylation of tectorigenin and tectoridin. To elucidate the catalytic mechanisms of Bc7OUGT, we solved its crystal structure in complex with UDP and UDP/tectorigenin respectively. Structural analysis revealed the Bc7OUGT possesses a narrow but novel substrate-binding pocket made up by plentiful aromatic residues. Further structure-guided mutagenesis of these residues increased both glycosylation and deglycosylation activities. The catalytic reversibility of Bc7OUGT was also successfully applied in an one-pot aglycon exchange reaction. Our findings demonstrated the promising biopesticide activity of tectorigenin and its glycosides, and the characterization and mechanistic study of Bc7OUGT could facilitate the design of novel reversible UGTs to produce valuable glycosides with health benefits for both plants and humans.

PubMed: 37581303
DOI: 10.1111/pbi.14157

実験手法

X-RAY DIFFRACTION (2.12 Å)