8IL9

Crystal structure of the LOV1 Q122N mutant of Klebsormidium nitens phototropin

8IL9 の概要

• エントリーDOI: 10.2210/pdb8il9/pdb
• 分子名称: Phototropin, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: blue light photoreceptor, flavoprotein
• 由来する生物種: Klebsormidium nitens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17452.64

構造登録者

Gautam, A.K.,Sharma, S.,Gourinath, S.,Kateriya, S. (登録日: 2023-03-03, 公開日: 2024-03-06, 最終更新日: 2024-12-18)

主引用文献

Sharma, S.,Gautam, A.K.,Singh, R.,Gourinath, S.,Kateriya, S.
Unusual photodynamic characteristics of the light-oxygen-voltage domain of phototropin linked to terrestrial adaptation of Klebsormidium nitens.
Febs J., 291:5156-5176, 2024

PubMed Abstract: Phototropin (Phot), a blue light-sensing LOV domain protein, mediates blue light responses and is evolutionarily conserved across the green lineage. Klebsormidium nitens, a green terrestrial alga, presents a valuable opportunity to study adaptive responses from aquatic to land habitat transitions. We determined the crystal structure of Klebsormidium nitens Phot LOV1 domain (KnLOV1) in the dark and engineered different mutations (R60K, Q122N, and D33N) to modulate the lifetime of the photorecovery cycle. We observed unusual, slow recovery kinetics in the wild-type KnLOV1 domain (τ = 41 ± 3 min) compared to different mutants (R60K: τ = 2.0 ± 0.1 min, Q122N: τ = 1.7 ± 0.1 min, D33N: τ = 9.6 ± 0.1 min). Crystal structures of wild-type KnLOV1 and mutants revealed subtle but critical changes near the protein chromophore that is responsible for modulating protein dark recovery time. Our findings shed light on the unique structural and biochemical characteristics of the newly studied KnLOV1 and its evolutionary importance for phototropin-mediated physiology.

PubMed: 39344087
DOI: 10.1111/febs.17284

実験手法

X-RAY DIFFRACTION (2.16 Å)