8II0
FACTOR INHIBITING HIF-1 ALPHA in complex with (5-(3-(3-chlorophenyl)isoxazol-5-yl)-3-hydroxypicolinoyl)glycine
Summary for 8II0
| Entry DOI | 10.2210/pdb8ii0/pdb |
| Descriptor | Hypoxia-inducible factor 1-alpha inhibitor, 2-[[5-[3-(3-chlorophenyl)-1,2-oxazol-5-yl]-3-oxidanyl-pyridin-2-yl]carbonylamino]ethanoic acid, GLYCEROL, ... (6 entities in total) |
| Functional Keywords | factor-inhibiting hypoxia-inducible factor, fih, 2og dependent dioxygenase, oxidoreductase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 41720.13 |
| Authors | Nakashima, Y.,Corner, T.,Zhang, X.,Schofield, C.J. (deposition date: 2023-02-24, release date: 2024-02-28, Last modification date: 2024-10-09) |
| Primary citation | Wu, Y.,Chen, Y.,Corner, T.P.,Nakashima, Y.,Salah, E.,Li, Z.,Zhang, L.,Yang, L.,Tumber, A.,Sun, Z.,Wen, Y.,Zhong, A.,Yang, F.,Li, X.,Zhang, Z.,Schofield, C.J.,Zhang, X. A Small-Molecule Inhibitor of Factor Inhibiting HIF Binding to a Tyrosine-Flip Pocket for the Treatment of Obesity. Angew.Chem.Int.Ed.Engl., 63:e202410438-e202410438, 2024 Cited by PubMed Abstract: In animals, limiting oxygen upregulates the hypoxia-inducible factor (HIF) and promotes a metabolic shift towards glycolysis. Factor inhibiting HIF (FIH) is an asparaginyl hydroxylase that regulates HIF function by reducing its interaction with histone acetyl transferases. HIF levels are negatively regulated by the HIF prolyl hydroxylases (PHDs) which, like FIH, are 2-oxoglutarate (2OG) oxygenases. Genetic loss of FIH promotes both glycolysis and aerobic metabolism. FIH has multiple non-HIF substrates making it challenging to connect its biochemistry with physiology. A structure-mechanism guided approach identified a highly potent in vivo active FIH inhibitor, ZG-2291, the binding of which promotes a conformational flip of a catalytically important tyrosine, enabling the selective inhibition of FIH over other Jumonji C subfamily 2OG oxygenases. Consistent with genetic studies, ZG-2291 promotes thermogenesis and ameliorates symptoms of obesity and metabolic dysfunction in ob/ob mice. The results reveal ZG-2291 as a useful probe for the physiological functions of FIH and identify FIH inhibition as a promising strategy for obesity treatment. PubMed: 38923188DOI: 10.1002/anie.202410438 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.04 Å) |
Structure validation
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