8IHL

Overlapping tri-nucleosome

8IHL の概要

• エントリーDOI: 10.2210/pdb8ihl/pdb
• 関連するPDBエントリー: 5GSE
• EMDBエントリー: 35448
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: nucleosome, complex, nuclear protein, nuclear protein-dna complex, nuclear protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 497087.62

構造登録者

Nishimura, M.,Fujii, T.,Tanaka, H.,Maehara, K.,Nozawa, K.,Takizawa, Y.,Ohkawa, Y.,Kurumizaka, H. (登録日: 2023-02-23, 公開日: 2024-01-17, 最終更新日: 2024-01-24)

主引用文献

Nishimura, M.,Fujii, T.,Tanaka, H.,Maehara, K.,Morishima, K.,Shimizu, M.,Kobayashi, Y.,Nozawa, K.,Takizawa, Y.,Sugiyama, M.,Ohkawa, Y.,Kurumizaka, H.
Genome-wide mapping and cryo-EM structural analyses of the overlapping tri-nucleosome composed of hexasome-hexasome-octasome moieties.
Commun Biol, 7:61-61, 2024

PubMed Abstract: The nucleosome is a fundamental unit of chromatin in which about 150 base pairs of DNA are wrapped around a histone octamer. The overlapping di-nucleosome has been proposed as a product of chromatin remodeling around the transcription start site, and previously found as a chromatin unit, in which about 250 base pairs of DNA continuously bind to the histone core composed of a hexamer and an octamer. In the present study, our genome-wide analysis of human cells suggests another higher nucleosome stacking structure, the overlapping tri-nucleosome, which wraps about 300-350 base-pairs of DNA in the region downstream of certain transcription start sites of actively transcribed genes. We determine the cryo-electron microscopy (cryo-EM) structure of the overlapping tri-nucleosome, in which three subnucleosome moieties, hexasome, hexasome, and octasome, are associated by short connecting DNA segments. Small angle X-ray scattering and coarse-grained molecular dynamics simulation analyses reveal that the cryo-EM structure of the overlapping tri-nucleosome may reflect its structure in solution. Our findings suggest that nucleosome stacking structures composed of hexasome and octasome moieties may be formed by nucleosome remodeling factors around transcription start sites for gene regulation.

PubMed: 38191828
DOI: 10.1038/s42003-023-05694-1

実験手法

ELECTRON MICROSCOPY (7.64 Å)