8IBY

Structure of R2 with 5'ORF

8IBY の概要

• エントリーDOI: 10.2210/pdb8iby/pdb
• EMDBエントリー: 35349
• 分子名称: 5'ORF RNA, Reverse transcriptase-like protein, ZINC ION (3 entities in total)
• 機能のキーワード: r2 complex, rna binding protein/rna, rna binding protein-rna complex
• 由来する生物種: Bombyx mori (domestic silkworm); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 196371.01

構造登録者

Deng, P.,Tan, S.,Wang, J.,Liu, J.J. (登録日: 2023-02-10, 公開日: 2023-09-20)

主引用文献

Deng, P.,Tan, S.Q.,Yang, Q.Y.,Fu, L.,Wu, Y.,Zhu, H.Z.,Sun, L.,Bao, Z.,Lin, Y.,Zhang, Q.C.,Wang, H.,Wang, J.,Liu, J.G.
Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon.
Cell, 186:2865-2879.e20, 2023

PubMed Abstract: Retroelements are the widespread jumping elements considered as major drivers for genome evolution, which can also be repurposed as gene-editing tools. Here, we determine the cryo-EM structures of eukaryotic R2 retrotransposon with ribosomal DNA target and regulatory RNAs. Combined with biochemical and sequencing analysis, we reveal two essential DNA regions, Drr and Dcr, required for recognition and cleavage. The association of 3' regulatory RNA with R2 protein accelerates the first-strand cleavage, blocks the second-strand cleavage, and initiates the reverse transcription starting from the 3'-tail. Removing 3' regulatory RNA by reverse transcription allows the association of 5' regulatory RNA and initiates the second-strand cleavage. Taken together, our work explains the DNA recognition and RNA supervised sequential retrotransposition mechanisms by R2 machinery, providing insights into the retrotransposon and application reprogramming.

PubMed: 37301196
DOI: 10.1016/j.cell.2023.05.032

実験手法

ELECTRON MICROSCOPY (3.47 Å)