8HZZ

GuApiGT (UGT79B74)

8HZZ の概要

• エントリーDOI: 10.2210/pdb8hzz/pdb
• 分子名称: apiosyltransferase, SULFATE ION (3 entities in total)
• 機能のキーワード: glycosyltransferase, plant protein
• 由来する生物種: Glycyrrhiza uralensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102281.81

構造登録者

Wang, H.T.,Wang, Z.L.,Li, F.D.,Ye, M. (登録日: 2023-01-10, 公開日: 2023-09-20, 最終更新日: 2024-04-03)

主引用文献

Wang, H.T.,Wang, Z.L.,Chen, K.,Yao, M.J.,Zhang, M.,Wang, R.S.,Zhang, J.H.,Agren, H.,Li, F.D.,Li, J.,Qiao, X.,Ye, M.
Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants.
Nat Commun, 14:6658-6658, 2023

PubMed Abstract: Apiose is a natural pentose containing an unusual branched-chain structure. Apiosides are bioactive natural products widely present in the plant kingdom. However, little is known on the key apiosylation reaction in the biosynthetic pathways of apiosides. In this work, we discover an apiosyltransferase GuApiGT from Glycyrrhiza uralensis. GuApiGT could efficiently catalyze 2″-O-apiosylation of flavonoid glycosides, and exhibits strict selectivity towards UDP-apiose. We further solve the crystal structure of GuApiGT, determine a key sugar-binding motif (RLGSDH) through structural analysis and theoretical calculations, and obtain mutants with altered sugar selectivity through protein engineering. Moreover, we discover 121 candidate apiosyltransferase genes from Leguminosae plants, and identify the functions of 4 enzymes. Finally, we introduce GuApiGT and its upstream genes into Nicotiana benthamiana, and complete de novo biosynthesis of a series of flavonoid apiosides. This work reports an efficient phenolic apiosyltransferase, and reveals mechanisms for its sugar donor selectivity.

PubMed: 37863881
DOI: 10.1038/s41467-023-42393-1

実験手法

X-RAY DIFFRACTION (2.2 Å)