8HW8

Crystal structure of Heterodera glycines chitinase 2 D129A/E131A mutant in complex with nodulation factor SmNF-V (C16:2, S)

8HW8 の概要

• エントリーDOI: 10.2210/pdb8hw8/pdb
• 分子名称: Chitinase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: chitinase, hydrolase
• 由来する生物種: Heterodera glycines (soybean cyst nematode)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41134.47

構造登録者

Chen, W.,Chen, Q.,Wang, D.,Yang, Q. (登録日: 2022-12-29, 公開日: 2024-06-05, 最終更新日: 2024-12-18)

主引用文献

Chen, W.,Wang, D.,Ke, S.,Cao, Y.,Xiang, W.,Guo, X.,Yang, Q.
A soybean cyst nematode suppresses microbial plant symbionts using a lipochitooligosaccharide-hydrolysing enzyme.
Nat Microbiol, 9:1993-2005, 2024

PubMed Abstract: Cyst nematodes are the most damaging species of plant-parasitic nematodes. They antagonize the colonization of beneficial microbial symbionts that are important for nutrient acquisition of plants. The molecular mechanism of the antagonism, however, remains elusive. Here, through biochemical combined with structural analysis, we reveal that Heterodera glycines, the most notorious soybean cyst nematode, suppresses symbiosis by secreting an enzyme named HgCht2 to hydrolyse the key symbiotic signalling molecules, lipochitooligosaccharides (LCOs). We solved the three-dimensional structures of apo HgCht2, as well as its chitooligosaccharide-bound and LCO-bound forms. These structures elucidated the substrate binding and hydrolysing mechanism of the enzyme. We designed an HgCht2 inhibitor, 1516b, which successfully suppresses the antagonism of cyst nematodes towards nitrogen-fixing rhizobia and phosphorus-absorbing arbuscular mycorrhizal symbioses. As HgCht2 is phylogenetically conserved across all cyst nematodes, our study revealed a molecular mechanism by which parasitic cyst nematodes antagonize the establishment of microbial symbiosis and provided a small-molecule solution.

PubMed: 38886584
DOI: 10.1038/s41564-024-01727-5

実験手法

X-RAY DIFFRACTION (1.57 Å)