8HVB

Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, lacto-N-biose complex

8HVB の概要

• エントリーDOI: 10.2210/pdb8hvb/pdb
• 分子名称: Lacto-N-biosidase, beta-D-galactopyranose, 2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: glycoside hydrolase, lacto-n-biosidase, lacto-n-biose complex, hydrolase
• 由来する生物種: Streptomyces sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66636.33

構造登録者

Fushinobu, S.,Yamada, C.,Fujio, N. (登録日: 2022-12-26, 公開日: 2023-12-27, 最終更新日: 2025-07-09)

主引用文献

Fujio, N.,Fushinobu, S.,Yamada, C.
Crystal Structure of Glycoside Hydrolase Family 20 Lacto- N -biosidase from Soil Bacterium Streptomyces sp. Strain 142.
J Appl Glycosci (1999), 72:7202101-7202101, 2025

PubMed Abstract: Lacto--biosidase hydrolyzes the β-GlcNAc or β-GalNAc bond of sugar chains to release lacto--biose I (Gal-β1,3-GlcNAc) or galacto--biose (Gal-β1,3-GalNAc) from the non-reducing end. Typical substrates for lacto--biosidase include type I oligosaccharides contained in human breast milk, such as lacto--tetraose. Lacto--biosidases have recently received significant attention because of their potential to synthesize milk oligosaccharides. Bifidobacterial lacto--biosidases belonging to glycoside hydrolase families 20 and 136 have been studied. The GH20 lacto--biosidases utilize a substrate-associated hydrolysis mechanism. LnbB from is the only lacto--biosidase with reported crystal structures in GH20. In this study, the crystal structure of the lacto--biosidase from sp. strain 142 (LNBase) was solved in a complex with lacto--biose and galacto--biose. The stabilizing residue, which recognizes the nitrogen atom of the -acetyl group of the -1 subsite, and the catalytic acid/base residue, were determined to be D304 and E305, respectively. The structure of LNBase is similar to that of LnbB; however, in the complex with galacto--biose, there were two structures exhibiting different sugar conformations. A phylogenetic analysis revealed that lacto--biosidases discovered in the soil bacteria spp. and human gut bacteria spp. may be divided into two separate groups, which suggests that they evolved divergently.

PubMed: 40502377
DOI: 10.5458/jag.7202101

実験手法

X-RAY DIFFRACTION (1.6 Å)