8HTY

Candida boidinii Formate Dehydrogenase Crystal Structure at 1.4 Angstrom Resolution

Summary for 8HTY

• Entry DOI: 10.2210/pdb8hty/pdb
• Descriptor: Formate dehydrogenase, SULFATE ION (3 entities in total)
• Functional Keywords: formate dehydrogenase, candida boidinii, oxidoreductase
• Biological source: [Candida] boidinii
• Total number of polymer chains: 4
• Total formula weight: 162804.76

Authors

Gul, M.,Yuksel, B.,Bulut, H.,DeMirci, H. (deposition date: 2022-12-22, release date: 2023-01-18, Last modification date: 2023-11-08)

Primary citation

Gul, M.,Yuksel, B.,Bulut, H.,DeMirci, H.
Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography.
Acta Crystallogr D Struct Biol, 79:1010-1017, 2023

PubMed Abstract: Candida boidinii NAD-dependent formate dehydrogenase (CbFDH) has gained significant attention for its potential application in the production of biofuels and various industrial chemicals from inorganic carbon dioxide. The present study reports the atomic X-ray crystal structures of wild-type CbFDH at cryogenic and ambient temperatures, as well as that of the Val120Thr mutant at cryogenic temperature, determined at the Turkish Light Source `Turkish DeLight'. The structures reveal new hydrogen bonds between Thr120 and water molecules in the active site of the mutant CbFDH, suggesting increased stability of the active site and more efficient electron transfer during the reaction. Further experimental data is needed to test these hypotheses. Collectively, these findings provide invaluable insights into future protein-engineering efforts that could potentially enhance the efficiency and effectiveness of CbFDH.

PubMed: 37860962
DOI: 10.1107/S2059798323008070

Experimental method

X-RAY DIFFRACTION (1.4 Å)