8HSL

Thermus thermophilus RNA polymerase bound with an inverted Rho hexamer

8HSL の概要

• エントリーDOI: 10.2210/pdb8hsl/pdb
• 関連するPDBエントリー: 8HSG 8HSH 8HSJ 8HSR
• EMDBエントリー: 34996 34997 34999 35000 35004
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: transcription termination, rna polymerase, transcription termination factor rho, transcription
• 由来する生物種: Thermus thermophilus HB8; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 670248.99

構造登録者

Murayama, Y.,Ehara, H.,Sekine, S. (登録日: 2022-12-19, 公開日: 2023-05-03, 最終更新日: 2024-07-03)

主引用文献

Murayama, Y.,Ehara, H.,Aoki, M.,Goto, M.,Yokoyama, T.,Sekine, S.I.
Structural basis of the transcription termination factor Rho engagement with transcribing RNA polymerase from Thermus thermophilus.
Sci Adv, 9:eade7093-eade7093, 2023

PubMed Abstract: Transcription termination is an essential step in transcription by RNA polymerase (RNAP) and crucial for gene regulation. For many bacterial genes, transcription termination is mediated by the adenosine triphosphate-dependent RNA translocase/helicase Rho, which causes RNA/DNA dissociation from the RNAP elongation complex (EC). However, the structural basis of the interplay between Rho and RNAP remains obscure. Here, we report the cryo-electron microscopy structure of the RNAP EC engaged with Rho. The Rho hexamer binds RNAP through the carboxyl-terminal domains, which surround the RNA exit site of RNAP, directing the nascent RNA seamlessly from the RNA exit to its central channel. The β-flap tip at the RNA exit is critical for the Rho-dependent RNA release, and its deletion causes an alternative Rho-RNAP binding mode, which is irrelevant to termination. The Rho binding site overlaps with the binding sites of other macromolecules, such as ribosomes, providing a general basis of gene regulation.

PubMed: 36753546
DOI: 10.1126/sciadv.ade7093

実験手法

ELECTRON MICROSCOPY (5.8 Å)