8HR9

Structure of tetradecameric RdrA ring

8HR9 の概要

• エントリーDOI: 10.2210/pdb8hr9/pdb
• EMDBエントリー: 34964
• 分子名称: Archaeal ATPase, ADENOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: cryoelectron microscopy, adenosine triphosphatase, immune system
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 1504927.13

構造登録者

Gao, Y. (登録日: 2022-12-15, 公開日: 2023-02-01, 最終更新日: 2023-08-16)

主引用文献

Gao, Y.,Luo, X.,Li, P.,Li, Z.,Ye, F.,Liu, S.,Gao, P.
Molecular basis of RADAR anti-phage supramolecular assemblies.
Cell, 186:999-1012.e20, 2023

PubMed Abstract: Adenosine-to-inosine RNA editing has been proposed to be involved in a bacterial anti-phage defense system called RADAR. RADAR contains an adenosine triphosphatase (RdrA) and an adenosine deaminase (RdrB). Here, we report cryo-EM structures of RdrA, RdrB, and currently identified RdrA-RdrB complexes in the presence or absence of RNA and ATP. RdrB assembles into a dodecameric cage with catalytic pockets facing outward, while RdrA adopts both autoinhibited tetradecameric and activation-competent heptameric rings. Structural and functional data suggest a model in which RNA is loaded through the bottom section of the RdrA ring and translocated along its inner channel, a process likely coupled with ATP-binding status. Intriguingly, up to twelve RdrA rings can dock one RdrB cage with precise alignments between deaminase catalytic pockets and RNA-translocation channels, indicative of enzymatic coupling of RNA translocation and deamination. Our data uncover an interesting mechanism of enzymatic coupling and anti-phage defense through supramolecular assemblies.

PubMed: 36764292
DOI: 10.1016/j.cell.2023.01.026

実験手法

ELECTRON MICROSCOPY (3.03 Å)