8HHM

Cryo-EM structure of the Cas12m2-crRNA-target DNA ternary complex intermediate state

Summary for 8HHM

Entry DOI	10.2210/pdb8hhm/pdb
EMDB information	34804
Descriptor	RNA (56-MER), DNA (36-MER), Cas12m2, ... (6 entities in total)
Functional Keywords	crispr-cas, rna binding protein-dna complex, rna binding protein
Biological source	Mycolicibacterium mucogenicum More
Total number of polymer chains	4
Total formula weight	107326.08
Authors	Omura, N.S.,Nakagawa, R.,Wu, Y.W.,Sudfeld, C.,Warren, V.R.,Hirano, H.,Kusakizako, T.,Kise, Y.,Lebbink, H.G.J.,Itoh, Y.,Oost, V.D.J.,Nureki, O. (deposition date: 2022-11-16, release date: 2023-04-12, Last modification date: 2024-05-22)
Primary citation	Omura, S.N.,Nakagawa, R.,Sudfeld, C.,Villegas Warren, R.,Wu, W.Y.,Hirano, H.,Laffeber, C.,Kusakizako, T.,Kise, Y.,Lebbink, J.H.G.,Itoh, Y.,van der Oost, J.,Nureki, O. Mechanistic and evolutionary insights into a type V-M CRISPR-Cas effector enzyme. Nat.Struct.Mol.Biol., 30:1172-1182, 2023 Cited by PubMed: 37460897 DOI: 10.1038/s41594-023-01042-3 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.08 Å)

Structure validation