8HF2

Cryo-EM structure of WeiTsing

8HF2 の概要

• エントリーDOI: 10.2210/pdb8hf2/pdb
• EMDBエントリー: 34710
• 分子名称: PRA1 family protein (1 entity in total)
• 機能のキーワード: pentamer, channel, membrane protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 80019.95

構造登録者

Qin, L.,Tang, L.H.,Chen, Y.H. (登録日: 2022-11-09, 公開日: 2023-06-21, 最終更新日: 2025-07-02)

主引用文献

Wang, W.,Qin, L.,Zhang, W.,Tang, L.,Zhang, C.,Dong, X.,Miao, P.,Shen, M.,Du, H.,Cheng, H.,Wang, K.,Zhang, X.,Su, M.,Lu, H.,Li, C.,Gao, Q.,Zhang, X.,Huang, Y.,Liang, C.,Zhou, J.M.,Chen, Y.H.
WeiTsing, a pericycle-expressed ion channel, safeguards the stele to confer clubroot resistance.
Cell, 186:2656-2671.e18, 2023

PubMed Abstract: Plant roots encounter numerous pathogenic microbes that often cause devastating diseases. One such pathogen, Plasmodiophora brassicae (Pb), causes clubroot disease and severe yield losses on cruciferous crops worldwide. Here, we report the isolation and characterization of WeiTsing (WTS), a broad-spectrum clubroot resistance gene from Arabidopsis. WTS is transcriptionally activated in the pericycle upon Pb infection to prevent pathogen colonization in the stele. Brassica napus carrying the WTS transgene displayed strong resistance to Pb. WTS encodes a small protein localized in the endoplasmic reticulum (ER), and its expression in plants induces immune responses. The cryoelectron microscopy (cryo-EM) structure of WTS revealed a previously unknown pentameric architecture with a central pore. Electrophysiology analyses demonstrated that WTS is a calcium-permeable cation-selective channel. Structure-guided mutagenesis indicated that channel activity is strictly required for triggering defenses. The findings uncover an ion channel analogous to resistosomes that triggers immune signaling in the pericycle.

PubMed: 37295403
DOI: 10.1016/j.cell.2023.05.023

実験手法

ELECTRON MICROSCOPY (4.14 Å)