8HBE

Structure of human soluble guanylate cyclase in the inactive state at 3.1 angstrom

8HBE の概要

• エントリーDOI: 10.2210/pdb8hbe/pdb
• EMDBエントリー: 34623
• 分子名称: Guanylate cyclase soluble subunit alpha-1, Guanylate cyclase soluble subunit beta-1, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: soluble guanylate cyclase, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 148782.29

構造登録者

Chen, L.,Liu, R. (登録日: 2022-10-28, 公開日: 2023-04-19, 最終更新日: 2025-06-18)

主引用文献

Liu, R.,Kang, Y.,Chen, L.
NO binds to the distal site of haem in the fully activated soluble guanylate cyclase.
Nitric Oxide, 134-135:17-22, 2023

PubMed Abstract: Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO). The binding of NO to the haem of sGC induces a large conformational change in the enzyme and activates its cyclase activity. However, whether NO binds to the proximal site or the distal site of haem in the fully activated state remains under debate. Here, we present cryo-EM maps of sGC in the NO-activated state at high resolutions, allowing the observation of the density of NO. These cryo-EM maps show the binding of NO to the distal site of haem in the NO-activated state.

PubMed: 36972843
DOI: 10.1016/j.niox.2023.03.002

実験手法

ELECTRON MICROSCOPY (3.2 Å)