8H9Y

Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion condition

8H9Y の概要

• エントリーDOI: 10.2210/pdb8h9y/pdb
• 分子名称: Ion transport protein, DODECYL-BETA-D-MALTOSIDE, CHAPSO, ... (6 entities in total)
• 機能のキーワード: ion channel, membrane protein
• 由来する生物種: Aliarcobacter butzleri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38577.04

構造登録者

Irie, K.,Oda, Y. (登録日: 2022-10-25, 公開日: 2023-07-26, 最終更新日: 2024-05-29)

主引用文献

Irie, K.,Oda, Y.,Sumikama, T.,Oshima, A.,Fujiyoshi, Y.
The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.
Nat Commun, 14:4236-4236, 2023

PubMed Abstract: Divalent cation block is observed in various tetrameric ion channels. For blocking, a divalent cation is thought to bind in the ion pathway of the channel, but such block has not yet been directly observed. So, the behaviour of these blocking divalent cations remains still uncertain. Here, we elucidated the mechanism of the divalent cation block by reproducing the blocking effect into NavAb, a well-studied tetrameric sodium channel. Our crystal structures of NavAb mutants show that the mutations increasing the hydrophilicity of the inner vestibule of the pore domain enable a divalent cation to stack on the ion pathway. Furthermore, non-equilibrium molecular dynamics simulation showed that the stacking calcium ion repel sodium ion at the bottom of the selectivity filter. These results suggest the primary process of the divalent cation block mechanism in tetrameric cation channels.

PubMed: 37454189
DOI: 10.1038/s41467-023-39987-0

実験手法

X-RAY DIFFRACTION (3.4 Å)