8H9C

Crystal structure of chemically modified E. coli ThrS catalytic domain 4

8H9C の概要

• エントリーDOI: 10.2210/pdb8h9c/pdb
• 分子名称: Threonine--tRNA ligase, N-(2,3-dihydroxybenzoyl)-4-(4-nitrophenyl)-L-threonine, ZINC ION, ... (4 entities in total)
• 機能のキーワード: threonine--trna ligase, ligase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48363.26

構造登録者

Qiao, H.,Xia, M.,Wang, J.,Fang, P. (登録日: 2022-10-25, 公開日: 2023-02-08, 最終更新日: 2023-10-25)

主引用文献

Qiao, H.,Xia, M.,Cheng, Y.,Zhou, J.,Zheng, L.,Li, W.,Wang, J.,Fang, P.
Tyrosine-targeted covalent inhibition of a tRNA synthetase aided by zinc ion.
Commun Biol, 6:107-107, 2023

PubMed Abstract: Aminoacyl-tRNA synthetases (AARSs), a family of essential protein synthesis enzymes, are attractive targets for drug development. Although several different types of AARS inhibitors have been identified, AARS covalent inhibitors have not been reported. Here we present five unusual crystal structures showing that threonyl-tRNA synthetase (ThrRS) is covalently inhibited by a natural product, obafluorin (OB). The residue forming a covalent bond with OB is a tyrosine in ThrRS active center, which is not commonly modified by covalent inhibitors. The two hydroxyl groups on the o-diphenol moiety of OB form two coordination bonds with the conserved zinc ion in the active center of ThrRS. Therefore, the β-lactone structure of OB can undergo ester exchange reaction with the phenolic group of the adjacent tyrosine to form a covalent bond between the compound and the enzyme, and allow its nitrobenzene structure to occupy the binding site of tRNA. In addition, when this tyrosine was replaced by a lysine or even a weakly nucleophilic arginine, similar bonds could also be formed. Our report of the mechanism of a class of AARS covalent inhibitor targeting multiple amino acid residues could facilitate approaches to drug discovery for cancer and infectious diseases.

PubMed: 36707692
DOI: 10.1038/s42003-023-04517-7

実験手法

X-RAY DIFFRACTION (2.15 Å)