8H85

Trans-3/4-proline-hydroxylase H11 with 3-hydroxyl-proline

8H85 の概要

• エントリーDOI: 10.2210/pdb8h85/pdb
• 分子名称: Phytanoyl-CoA dioxygenase, 3-HYDROXYPROLINE (3 entities in total)
• 機能のキーワード: l-proline, trans, hydroxylase, akg, hydrolase, structural protein
• 由来する生物種: uncultured bacterium esnapd13
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30239.92

構造登録者

Gong, W.M.,Hu, X.Y. (登録日: 2022-10-21, 公開日: 2023-04-19, 最終更新日: 2023-11-29)

主引用文献

Hu, X.,Huang, X.,Liu, J.,Zheng, P.,Gong, W.,Yang, L.
Structures of L-proline trans-hydroxylase reveal the catalytic specificity and provide deeper insight into AKG-dependent hydroxylation.
Acta Crystallogr D Struct Biol, 79:318-325, 2023

PubMed Abstract: L-Proline hydroxylase is a member of the non-heme Fe/α-ketoglutarate (AKG)-dependent hydroxylase family that catalyzes the reaction from L-proline to hydroxy-L-proline, which is widely used in drug synthesis, biochemistry, food supplementation and cosmetic industries. Here, the first crystal structure of L-proline trans-hydroxylase and its complexes with substrate and product are reported, which reveal the structural basis of trans-cis proline hydroxylation selectivity. Structure comparison with other AKG-dependent hydroxylases identifies conserved amino acid residues, which may serve as signatures of in-line or off-line AKG binding modes in the AKG-dependent enzyme family.

PubMed: 36974966
DOI: 10.1107/S2059798323001936

実験手法

X-RAY DIFFRACTION (2.38 Å)