8H79

The crystal structure of cyanorhodopsin-II (CyR-II) P7104R from Nodosilinea nodulosa PCC 7104

8H79 の概要

• エントリーDOI: 10.2210/pdb8h79/pdb
• 分子名称: cyanorhodopsin-II (CyR-II) P7104R, RETINAL, TETRAETHYLENE GLYCOL, ... (10 entities in total)
• 機能のキーワード: retinal cell-free synthesis bacterial type rhodopsin cyanobacteria, membrane protein
• 由来する生物種: Nodosilinea nodulosa PCC 7104
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30251.85

構造登録者

Hosaka, T.,Kimura-Someya, T.,Shirouzu, M. (登録日: 2022-10-19, 公開日: 2023-10-25, 最終更新日: 2024-11-13)

主引用文献

Hasegawa-Takano, M.,Hosaka, T.,Kojima, K.,Nishimura, Y.,Kurihara, M.,Nakajima, Y.,Ishizuka-Katsura, Y.,Kimura-Someya, T.,Shirouzu, M.,Sudo, Y.,Yoshizawa, S.
Cyanorhodopsin-II represents a yellow-absorbing proton-pumping rhodopsin clade within cyanobacteria.
Isme J, 18:-, 2024

PubMed Abstract: Microbial rhodopsins are prevalent in many cyanobacterial groups as a light-energy-harvesting system in addition to the photosynthetic system. It has been suggested that this dual system allows efficient capture of sunlight energy using complementary ranges of absorption wavelengths. However, the diversity of cyanobacterial rhodopsins, particularly in accumulated metagenomic data, remains underexplored. Here, we used a metagenomic mining approach, which led to the identification of a novel rhodopsin clade unique to cyanobacteria, cyanorhodopsin-II (CyR-II). CyR-IIs function as light-driven outward H+ pumps. CyR-IIs, together with previously identified cyanorhodopsins (CyRs) and cyanobacterial halorhodopsins (CyHRs), constitute cyanobacterial ion-pumping rhodopsins (CyipRs), a phylogenetically distinct family of rhodopsins. The CyR-II clade is further divided into two subclades, YCyR-II and GCyR-II, based on their specific absorption wavelength. YCyR-II absorbed yellow light (λmax = 570 nm), whereas GCyR-II absorbed green light (λmax = 550 nm). X-ray crystallography and mutational analysis revealed that the difference in absorption wavelengths is attributable to slight changes in the side chain structure near the retinal chromophore. The evolutionary trajectory of cyanobacterial rhodopsins suggests that the function and light-absorbing range of these rhodopsins have been adapted to a wide range of habitats with variable light and environmental conditions. Collectively, these findings shed light on the importance of rhodopsins in the evolution and environmental adaptation of cyanobacteria.

PubMed: 39485071
DOI: 10.1093/ismejo/wrae175

実験手法

X-RAY DIFFRACTION (2.07 Å)