8H6E
Cryo-EM structure of human exon-defined spliceosome in the late pre-B state.
This is a non-PDB format compatible entry.
Summary for 8H6E
| Entry DOI | 10.2210/pdb8h6e/pdb |
| EMDB information | 34500 |
| Descriptor | pre-mRNA, U5 snRNA, Pre-mRNA-processing-splicing factor 8, ... (51 entities in total) |
| Functional Keywords | exon-defined spliceosome, late pre-b state, rna splicing, splicing |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 61 |
| Total formula weight | 2736775.67 |
| Authors | |
| Primary citation | Zhang, W.,Zhang, X.,Zhan, X.,Bai, R.,Lei, J.,Yan, C.,Shi, Y. Structural insights into human exon-defined spliceosome prior to activation. Cell Res., 34:428-439, 2024 Cited by PubMed Abstract: Spliceosome is often assembled across an exon and undergoes rearrangement to span a neighboring intron. Most states of the intron-defined spliceosome have been structurally characterized. However, the structure of a fully assembled exon-defined spliceosome remains at large. During spliceosome assembly, the pre-catalytic state (B complex) is converted from its precursor (pre-B complex). Here we report atomic structures of the exon-defined human spliceosome in four sequential states: mature pre-B, late pre-B, early B, and mature B. In the previously unknown late pre-B state, U1 snRNP is already released but the remaining proteins are still in the pre-B state; unexpectedly, the RNAs are in the B state, with U6 snRNA forming a duplex with 5'-splice site and U5 snRNA recognizing the 3'-end of the exon. In the early and mature B complexes, the B-specific factors are stepwise recruited and specifically recognize the exon 3'-region. Our study reveals key insights into the assembly of the exon-defined spliceosomes and identifies mechanistic steps of the pre-B-to-B transition. PubMed: 38658629DOI: 10.1038/s41422-024-00949-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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