8H6E

Cryo-EM structure of human exon-defined spliceosome in the late pre-B state.

これはPDB形式変換不可エントリーです。

8H6E の概要

• エントリーDOI: 10.2210/pdb8h6e/pdb
• EMDBエントリー: 34500
• 分子名称: pre-mRNA, U5 snRNA, Pre-mRNA-processing-splicing factor 8, ... (51 entities in total)
• 機能のキーワード: exon-defined spliceosome, late pre-b state, rna splicing, splicing
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 61
• 化学式量合計: 2736775.67

構造登録者

Zhang, W.,Zhan, X.,Zhang, X.,Bai, R.,Lei, J.,Yan, C.,Shi, Y. (登録日: 2022-10-17, 公開日: 2024-05-01, 最終更新日: 2024-06-12)

主引用文献

Zhang, W.,Zhang, X.,Zhan, X.,Bai, R.,Lei, J.,Yan, C.,Shi, Y.
Structural insights into human exon-defined spliceosome prior to activation.
Cell Res., 34:428-439, 2024

PubMed Abstract: Spliceosome is often assembled across an exon and undergoes rearrangement to span a neighboring intron. Most states of the intron-defined spliceosome have been structurally characterized. However, the structure of a fully assembled exon-defined spliceosome remains at large. During spliceosome assembly, the pre-catalytic state (B complex) is converted from its precursor (pre-B complex). Here we report atomic structures of the exon-defined human spliceosome in four sequential states: mature pre-B, late pre-B, early B, and mature B. In the previously unknown late pre-B state, U1 snRNP is already released but the remaining proteins are still in the pre-B state; unexpectedly, the RNAs are in the B state, with U6 snRNA forming a duplex with 5'-splice site and U5 snRNA recognizing the 3'-end of the exon. In the early and mature B complexes, the B-specific factors are stepwise recruited and specifically recognize the exon 3'-region. Our study reveals key insights into the assembly of the exon-defined spliceosomes and identifies mechanistic steps of the pre-B-to-B transition.

PubMed: 38658629
DOI: 10.1038/s41422-024-00949-w

実験手法

ELECTRON MICROSCOPY (3.2 Å)