8GXO

The crystal structure of CsFAOMT1 in complex with SAH

8GXO の概要

• エントリーDOI: 10.2210/pdb8gxo/pdb
• 分子名称: Caffeoyl-CoA O-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: csfaomt1, sah, o-methyltransferase, transferase
• 由来する生物種: Camellia sinensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54334.70

構造登録者

Zhang, Z.M.,Zhou, Y.E.,Huang, H.S. (登録日: 2022-09-20, 公開日: 2023-09-13, 最終更新日: 2026-03-11)

主引用文献

Jin, J.Q.,Qu, F.R.,Huang, H.,Liu, Q.S.,Wei, M.Y.,Zhou, Y.,Huang, K.L.,Cui, Z.,Chen, J.D.,Dai, W.D.,Zhu, L.,Yao, M.Z.,Zhang, Z.M.,Chen, L.
Characterization of two O-methyltransferases involved in the biosynthesis of O-methylated catechins in tea plant.
Nat Commun, 14:5075-5075, 2023

PubMed Abstract: Tea is known for having a high catechin content, with the main component being (-)-epigallocatechin gallate (EGCG), which has significant bioactivities, including potential anti-cancer and anti-inflammatory activity. The poor intestinal stability and permeability of EGCG, however, undermine these health-improving benefits. O-methylated EGCG derivatives, found in a few tea cultivars in low levels, have attracted considerable interest due to their increased bioavailability. Here, we identify two O-methyltransferases from tea plant: CsFAOMT1 that has a specific O-methyltransferase activity on the 3''-position of EGCG to generate EGCG3''Me, and CsFAOMT2 that predominantly catalyzes the formation of EGCG4″Me. In different tea tissues and germplasms, the transcript levels of CsFAOMT1 and CsFAOMT2 are strongly correlated with the amounts of EGCG3''Me and EGCG4''Me, respectively. Furthermore, the crystal structures of CsFAOMT1 and CsFAOMT2 reveal the key residues necessary for 3''- and 4''-O-methylation. These findings may provide guidance for the future development of tea cultivars with high O-methylated catechin content.

PubMed: 37604798
DOI: 10.1038/s41467-023-40868-9

実験手法

X-RAY DIFFRACTION (1.74 Å)