8GKG

Human TRPV3 pentamer structure

Summary for 8GKG

• Entry DOI: 10.2210/pdb8gkg/pdb
• Related: 8GKA
• EMDB information: 40181 40183
• Descriptor: Transient receptor potential cation channel subfamily V member 3 (1 entity in total)
• Functional Keywords: trp channel, ion channel, pore dilation, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 5
• Total formula weight: 453714.06

Authors

Lansky, S.,Betancourt, J.M.,Scheuring, S. (deposition date: 2023-03-18, release date: 2023-09-06, Last modification date: 2023-09-20)

Primary citation

Lansky, S.,Betancourt, J.M.,Zhang, J.,Jiang, Y.,Kim, E.D.,Paknejad, N.,Nimigean, C.M.,Yuan, P.,Scheuring, S.
A pentameric TRPV3 channel with a dilated pore.
Nature, 621:206-214, 2023

PubMed Abstract: Transient receptor potential (TRP) channels are a large, eukaryotic ion channel superfamily that control diverse physiological functions, and therefore are attractive drug targets. More than 210 structures from more than 20 different TRP channels have been determined, and all are tetramers. Despite this wealth of structures, many aspects concerning TRPV channels remain poorly understood, including the pore-dilation phenomenon, whereby prolonged activation leads to increased conductance, permeability to large ions and loss of rectification. Here, we used high-speed atomic force microscopy (HS-AFM) to analyse membrane-embedded TRPV3 at the single-molecule level and discovered a pentameric state. HS-AFM dynamic imaging revealed transience and reversibility of the pentamer in dynamic equilibrium with the canonical tetramer through membrane diffusive protomer exchange. The pentamer population increased upon diphenylboronic anhydride (DPBA) addition, an agonist that has been shown to induce TRPV3 pore dilation. On the basis of these findings, we designed a protein production and data analysis pipeline that resulted in a cryogenic-electron microscopy structure of the TRPV3 pentamer, showing an enlarged pore compared to the tetramer. The slow kinetics to enter and exit the pentameric state, the increased pentamer formation upon DPBA addition and the enlarged pore indicate that the pentamer represents the structural correlate of pore dilation. We thus show membrane diffusive protomer exchange as an additional mechanism for structural changes and conformational variability. Overall, we provide structural evidence for a non-canonical pentameric TRP-channel assembly, laying the foundation for new directions in TRP channel research.

PubMed: 37648856
DOI: 10.1038/s41586-023-06470-1

Experimental method

ELECTRON MICROSCOPY (4.38 Å)