8GKA

Human TRPV3 tetramer structure, closed conformation

8GKA の概要

• エントリーDOI: 10.2210/pdb8gka/pdb
• EMDBエントリー: 40181
• 分子名称: Transient receptor potential cation channel subfamily V member 3, SODIUM ION, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (3 entities in total)
• 機能のキーワード: ion channel, trp channel, closed conformation, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 388076.75

構造登録者

Lansky, S.,Betancourt, J.M.,Scheuring, S. (登録日: 2023-03-17, 公開日: 2023-09-06, 最終更新日: 2024-11-06)

主引用文献

Lansky, S.,Betancourt, J.M.,Zhang, J.,Jiang, Y.,Kim, E.D.,Paknejad, N.,Nimigean, C.M.,Yuan, P.,Scheuring, S.
A pentameric TRPV3 channel with a dilated pore.
Nature, 621:206-214, 2023

PubMed Abstract: Transient receptor potential (TRP) channels are a large, eukaryotic ion channel superfamily that control diverse physiological functions, and therefore are attractive drug targets. More than 210 structures from more than 20 different TRP channels have been determined, and all are tetramers. Despite this wealth of structures, many aspects concerning TRPV channels remain poorly understood, including the pore-dilation phenomenon, whereby prolonged activation leads to increased conductance, permeability to large ions and loss of rectification. Here, we used high-speed atomic force microscopy (HS-AFM) to analyse membrane-embedded TRPV3 at the single-molecule level and discovered a pentameric state. HS-AFM dynamic imaging revealed transience and reversibility of the pentamer in dynamic equilibrium with the canonical tetramer through membrane diffusive protomer exchange. The pentamer population increased upon diphenylboronic anhydride (DPBA) addition, an agonist that has been shown to induce TRPV3 pore dilation. On the basis of these findings, we designed a protein production and data analysis pipeline that resulted in a cryogenic-electron microscopy structure of the TRPV3 pentamer, showing an enlarged pore compared to the tetramer. The slow kinetics to enter and exit the pentameric state, the increased pentamer formation upon DPBA addition and the enlarged pore indicate that the pentamer represents the structural correlate of pore dilation. We thus show membrane diffusive protomer exchange as an additional mechanism for structural changes and conformational variability. Overall, we provide structural evidence for a non-canonical pentameric TRP-channel assembly, laying the foundation for new directions in TRP channel research.

PubMed: 37648856
DOI: 10.1038/s41586-023-06470-1

実験手法

ELECTRON MICROSCOPY (2.55 Å)