8FX6

Non-ribosomal PCP-C didomain (amide stabilised leucine) acceptor bound state

8FX6 の概要

• エントリーDOI: 10.2210/pdb8fx6/pdb
• 分子名称: PCP-C didomain, N-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]-L-leucinamide (3 entities in total)
• 機能のキーワード: nrps, c-domain, pcp-domain, biosynthetic protein
• 由来する生物種: Thermobifida fusca
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 114487.25

構造登録者

Ho, Y.T.C.,Cryle, M.J. (登録日: 2023-01-24, 公開日: 2023-06-21, 最終更新日: 2024-11-13)

主引用文献

Ho, Y.T.C.,Kaczmarski, J.A.,Tailhades, J.,Izore, T.,Steer, D.L.,Schittenhelm, R.B.,Tosin, M.,Jackson, C.J.,Cryle, M.J.
Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis.
Chem.Commun.(Camb.), 59:8234-8237, 2023

PubMed Abstract: Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.

PubMed: 37310188
DOI: 10.1039/d3cc01578e

実験手法

X-RAY DIFFRACTION (2.2 Å)