8FWE

Neck structure of Agrobacterium phage Milano, C3 symmetry

これはPDB形式変換不可エントリーです。

8FWE の概要

• エントリーDOI: 10.2210/pdb8fwe/pdb
• 関連するPDBエントリー: 8FOP 8FOU 8FOY 8FQC 8FWB 8FWC
• EMDBエントリー: 29503
• 分子名称: Collar sheath protein, gp13, Portal protein, gp7, Neck 2 protein, gp15, ... (6 entities in total)
• 機能のキーワード: myophage, redox trigger, virus
• 由来する生物種: Agrobacterium phage Milano; 詳細
• タンパク質・核酸の鎖数: 102
• 化学式量合計: 2592545.44

構造登録者

Sonani, R.R.,Wang, F.,Esteves, N.C.,Kelly, R.J.,Sebastian, A.,Kreutzberger, M.A.B.,Leiman, P.G.,Scharf, B.E.,Egelman, E.H. (登録日: 2023-01-21, 公開日: 2023-12-06, 最終更新日: 2024-11-06)

主引用文献

Sonani, R.R.,Esteves, N.C.,Horton, A.A.,Kelly, R.J.,Sebastian, A.L.,Wang, F.,Kreutzberger, M.A.B.,Leiman, P.G.,Scharf, B.E.,Egelman, E.H.
Neck and capsid architecture of the robust Agrobacterium phage Milano.
Commun Biol, 6:921-921, 2023

PubMed Abstract: Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.

PubMed: 37684529
DOI: 10.1038/s42003-023-05292-1

実験手法

ELECTRON MICROSCOPY (3.46 Å)