8FVW

CryoEM structure of E.coli transcription elongation complex bound to ppGpp

8FVW の概要

• エントリーDOI: 10.2210/pdb8fvw/pdb
• 関連するPDBエントリー: 8FVW
• EMDBエントリー: 29491 29494
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, SODIUM ION, ... (12 entities in total)
• 機能のキーワード: transcription elongation, second messager, ppgpp, dna repair, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 429203.35

構造登録者

Duan, W.,Serganov, A. (登録日: 2023-01-19, 公開日: 2023-04-05, 最終更新日: 2024-06-19)

主引用文献

Weaver, J.W.,Proshkin, S.,Duan, W.,Epshtein, V.,Gowder, M.,Bharati, B.K.,Afanaseva, E.,Mironov, A.,Serganov, A.,Nudler, E.
Control of transcription elongation and DNA repair by alarmone ppGpp.
Nat.Struct.Mol.Biol., 30:600-607, 2023

PubMed Abstract: Second messenger (p)ppGpp (collectively guanosine tetraphosphate and guanosine pentaphosphate) mediates bacterial adaptation to nutritional stress by modulating transcription initiation. More recently, ppGpp has been implicated in coupling transcription and DNA repair; however, the mechanism of ppGpp engagement remained elusive. Here we present structural, biochemical and genetic evidence that ppGpp controls Escherichia coli RNA polymerase (RNAP) during elongation via a specific site that is nonfunctional during initiation. Structure-guided mutagenesis renders the elongation (but not initiation) complex unresponsive to ppGpp and increases bacterial sensitivity to genotoxic agents and ultraviolet radiation. Thus, ppGpp binds RNAP at sites with distinct functions in initiation and elongation, with the latter being important for promoting DNA repair. Our data provide insights on the molecular mechanism of ppGpp-mediated adaptation during stress, and further highlight the intricate relationships between genome stability, stress responses and transcription.

PubMed: 36997761
DOI: 10.1038/s41594-023-00948-2

実験手法

ELECTRON MICROSCOPY (2.1 Å)