8FVH

Pseudomonas phage E217 neck (portal, head-to-tail connector, collar and gateway proteins)

This is a non-PDB format compatible entry.

Summary for 8FVH

• Entry DOI: 10.2210/pdb8fvh/pdb
• EMDB information: 29406 29481 29486 29487
• Descriptor: E217 collar protein gp28, E217 gateway protein gp29, E217 head-to-tail connector protein gp27, ... (4 entities in total)
• Functional Keywords: pseudomonas, phage, e217, virus
• Biological source: Pseudomonas phage vB_PaeM_E217; More
• Total number of polymer chains: 36
• Total formula weight: 998004.91

Authors

Li, F.,Cingolani, G.,Hou, C. (deposition date: 2023-01-18, release date: 2023-07-26, Last modification date: 2025-05-14)

Primary citation

Li, F.,Hou, C.D.,Lokareddy, R.K.,Yang, R.,Forti, F.,Briani, F.,Cingolani, G.
High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.
Nat Commun, 14:4052-4052, 2023

PubMed Abstract: E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.

PubMed: 37422479
DOI: 10.1038/s41467-023-39756-z

Experimental method

ELECTRON MICROSCOPY (3.1 Å)