8FOJ

Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the post RNA handoff state

8FOJ の概要

• エントリーDOI: 10.2210/pdb8foj/pdb
• EMDBエントリー: 29351
• 分子名称: DNA polymerase, DNA primase, DNA primase large subunit, ... (7 entities in total)
• 機能のキーワード: dna polymerase, primase, dna replication, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 362919.45

構造登録者

Yuan, Z.,Georgescu, R.,Li, H.,O'Donnell, M. (登録日: 2022-12-30, 公開日: 2023-05-31, 最終更新日: 2024-03-13)

主引用文献

Yuan, Z.,Georgescu, R.,Li, H.,O'Donnell, M.E.
Molecular choreography of primer synthesis by the eukaryotic Pol alpha-primase.
Nat Commun, 14:3697-3697, 2023

PubMed Abstract: The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20-30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively. It has been unclear how Pol α hands over an RNA primer from Pri1 to Pol1 for DNA primer extension, and how the primer length is defined. Here we report the cryo-EM analysis of yeast Pol α in the apo, primer initiation, primer elongation, RNA primer hand-off from Pri1 to Pol1, and DNA extension states, revealing a series of very large movements. We reveal a critical point at which Pol1-core moves to take over the 3'-end of the RNA from Pri1. DNA extension is limited by a spiral motion of Pol1-core. Since both Pri1 and Pol1-core are flexibly attached to a stable platform, primer growth produces stress that limits the primer length.

PubMed: 37344454
DOI: 10.1038/s41467-023-39441-1

実験手法

ELECTRON MICROSCOPY (4.8 Å)