8FN4

Cryo-EM structure of RNase-treated RESC-A in trypanosomal RNA editing

8FN4 の概要

• エントリーDOI: 10.2210/pdb8fn4/pdb
• EMDBエントリー: 29305
• 分子名称: RNA-editing substrate-binding complex protein 1 (RESC1), RNA-editing substrate-binding complex protein 2 (RESC2), RNA-editing substrate-binding complex protein 3 (RESC3), ... (6 entities in total)
• 機能のキーワード: heat repeat, trypanosoma, rna editing substrate binding complex, grna, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Trypanosoma brucei; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 383422.30

構造登録者

Liu, S.,Wang, H.,Li, X.,Zhang, F.,Lee, J.K.J.,Li, Z.,Yu, C.,Zhao, X.,Hu, J.J.,Suematsu, T.,Alvarez-Cabrera, A.L.,Liu, Q.,Zhang, L.,Huang, L.,Aphasizheva, I.,Aphasizhev, R.,Zhou, Z.H. (登録日: 2022-12-26, 公開日: 2023-07-19, 最終更新日: 2024-06-19)

主引用文献

Liu, S.,Wang, H.,Li, X.,Zhang, F.,Lee, J.K.J.,Li, Z.,Yu, C.,Hu, J.J.,Zhao, X.,Suematsu, T.,Alvarez-Cabrera, A.L.,Liu, Q.,Zhang, L.,Huang, L.,Aphasizheva, I.,Aphasizhev, R.,Zhou, Z.H.
Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.
Science, 381:eadg4725-eadg4725, 2023

PubMed Abstract: In , the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and gRNA-mRNA-binding RESC-B and RESC-C particles. RESC-A sequesters gRNA termini, thus promoting hairpin formation and blocking mRNA access. The conversion of RESC-A into RESC-B or -C unfolds gRNA and allows mRNA selection. The ensuing gRNA-mRNA duplex protrudes from RESC-B, likely exposing editing sites to RECC-catalyzed cleavage, uridine insertion or deletion, and ligation. Our work reveals a remodeling event facilitating gRNA-mRNA hybridization and assembly of a macromolecular substrate for the editosome's catalytic modality.

PubMed: 37410820
DOI: 10.1126/science.adg4725

実験手法

ELECTRON MICROSCOPY (3.7 Å)