8FMX

Langya virus F glycoprotein ectodomain in prefusion form

8FMX の概要

• エントリーDOI: 10.2210/pdb8fmx/pdb
• EMDBエントリー: 29299 29300
• 分子名称: Fusion glycoprotein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: f ectodomain, prefusion, viral protein
• 由来する生物種: Langya virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 159316.63

構造登録者

Low, Y.S.,Isaacs, A.,Modhiran, N.,Watterson, D. (登録日: 2022-12-26, 公開日: 2023-06-28, 最終更新日: 2024-11-06)

主引用文献

Isaacs, A.,Low, Y.S.,Macauslane, K.L.,Seitanidou, J.,Pegg, C.L.,Cheung, S.T.M.,Liang, B.,Scott, C.A.P.,Landsberg, M.J.,Schulz, B.L.,Chappell, K.J.,Modhiran, N.,Watterson, D.
Structure and antigenicity of divergent Henipavirus fusion glycoproteins.
Nat Commun, 14:3577-3577, 2023

PubMed Abstract: In August 2022, a novel henipavirus (HNV) named Langya virus (LayV) was isolated from patients with severe pneumonic disease in China. This virus is closely related to Mòjiāng virus (MojV), and both are divergent from the bat-borne HNV members, Nipah (NiV) and Hendra (HeV) viruses. The spillover of LayV is the first instance of a HNV zoonosis to humans outside of NiV and HeV, highlighting the continuing threat this genus poses to human health. In this work, we determine the prefusion structures of MojV and LayV F proteins via cryogenic electron microscopy to 2.66 and 3.37 Å, respectively. We show that despite sequence divergence from NiV, the F proteins adopt an overall similar structure but are antigenically distinct as they do not react to known antibodies or sera. Glycoproteomic analysis revealed that while LayV F is less glycosylated than NiV F, it contains a glycan that shields a site of vulnerability previously identified for NiV. These findings explain the distinct antigenic profile of LayV and MojV F, despite the extent to which they are otherwise structurally similar to NiV. Our results carry implications for broad-spectrum HNV vaccines and therapeutics, and indicate an antigenic, yet not structural, divergence from prototypical HNVs.

PubMed: 37328468
DOI: 10.1038/s41467-023-39278-8

実験手法

ELECTRON MICROSCOPY (3.37 Å)