8FLX

De novo designed homotrimer; the fusion product of BGL17 and DHR59

8FLX の概要

• エントリーDOI: 10.2210/pdb8flx/pdb
• 分子名称: LK031 (1 entity in total)
• 機能のキーワード: de novo, computationally designed, designed, tandem repeat protein, de novo protein, kibloid, homotrimer
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38677.87

構造登録者

Kibler, R.D.,Kennedy, M.A.,Stoddard, B.L.,Lee, S. (登録日: 2022-12-22, 公開日: 2023-06-28, 最終更新日: 2025-01-01)

主引用文献

Lee, S.,Kibler, R.D.,Ahn, G.,Hsia, Y.,Borst, A.J.,Philomin, A.,Kennedy, M.A.,Huang, B.,Stoddard, B.,Baker, D.
Four-component protein nanocages designed by programmed symmetry breaking.
Nature, 2024

PubMed Abstract: Four, eight or twenty C3 symmetric protein trimers can be arranged with tetrahedral, octahedral or icosahedral point group symmetry to generate closed cage-like structures. Viruses access more complex higher triangulation number icosahedral architectures by breaking perfect point group symmetry, but nature appears not to have explored similar symmetry breaking for tetrahedral or octahedral symmetries. Here we describe a general design strategy for building higher triangulation number architectures starting from regular polyhedra through pseudosymmetrization of trimeric building blocks. Electron microscopy confirms the structures of T = 4 cages with 48 (tetrahedral), 96 (octahedral) and 240 (icosahedral) subunits, each with 4 distinct chains and 6 different protein-protein interfaces, and diameters of 33 nm, 43 nm and 75 nm, respectively. Higher triangulation number viruses possess very sophisticated functionalities; our general route to higher triangulation number nanocages should similarly enable a next generation of multiple antigen-displaying vaccine candidates and targeted delivery vehicles.

PubMed: 39695226
DOI: 10.1038/s41586-024-07814-1

実験手法

X-RAY DIFFRACTION (4.5 Å)