8FLC

Human nuclear pre-60S ribosomal subunit (State K3)

これはPDB形式変換不可エントリーです。

8FLC の概要

• エントリーDOI: 10.2210/pdb8flc/pdb
• EMDBエントリー: 29124 29202 29204 29274
• 分子名称: 60S ribosomal protein L12, 60S ribosomal protein L17, 60S ribosomal protein L18, ... (54 entities in total)
• 機能のキーワード: pre-60s ribosomal subunit, assembly intermediate, ribosome, nucleoprotein complex
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 50
• 化学式量合計: 2732553.12

構造登録者

Vanden Broeck, A.,Klinge, S. (登録日: 2022-12-21, 公開日: 2023-07-12, 最終更新日: 2023-07-26)

主引用文献

Vanden Broeck, A.,Klinge, S.
Principles of human pre-60 S biogenesis.
Science, 381:eadh3892-eadh3892, 2023

PubMed Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

PubMed: 37410842
DOI: 10.1126/science.adh3892

実験手法

ELECTRON MICROSCOPY (2.76 Å)