8FCK

Structure of the vertebrate augmin complex

8FCK の概要

• エントリーDOI: 10.2210/pdb8fck/pdb
• EMDBエントリー: 28981
• 分子名称: HAUS augmin-like complex subunit 1, HAUS augmin-like complex subunit 3, HAUS augmin like complex subunit 4 L homeolog, ... (8 entities in total)
• 機能のキーワード: microtubule, branching microtubule nucleation, spindle assembly, cell cycle
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 404339.49

構造登録者

Travis, S.M.,Huang, W.,Zhang, R.,Petry, S. (登録日: 2022-12-01, 公開日: 2023-04-19, 最終更新日: 2025-05-28)

主引用文献

Travis, S.M.,Mahon, B.P.,Huang, W.,Ma, M.,Rale, M.J.,Kraus, J.,Taylor, D.J.,Zhang, R.,Petry, S.
Integrated model of the vertebrate augmin complex.
Nat Commun, 14:2072-2072, 2023

PubMed Abstract: Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin's structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation.

PubMed: 37055408
DOI: 10.1038/s41467-023-37519-4

実験手法

ELECTRON MICROSCOPY (6.88 Å)