8FCG

Cryo-EM structure of Chikungunya virus asymmetric unit

これはPDB形式変換不可エントリーです。

8FCG の概要

• エントリーDOI: 10.2210/pdb8fcg/pdb
• EMDBエントリー: 28979
• 分子名称: E1 glycoprotein, E2 glycoprotein, Capsid protein, ... (4 entities in total)
• 機能のキーワード: chikv asymmetric unit, virus
• 由来する生物種: Chikungunya virus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 447952.53

構造登録者

Su, G.C.,Chmielewsk, D.,Kaelber, J.,Pintilie, G.,Chen, M.,Jin, J.,Auguste, A.,Chiu, W. (登録日: 2022-12-01, 公開日: 2024-03-20, 最終更新日: 2024-04-03)

主引用文献

Chmielewski, D.,Su, G.C.,Kaelber, J.T.,Pintilie, G.D.,Chen, M.,Jin, J.,Auguste, A.J.,Chiu, W.
Cryogenic electron microscopy and tomography reveal imperfect icosahedral symmetry in alphaviruses.
Pnas Nexus, 3:pgae102-pgae102, 2024

PubMed Abstract: Alphaviruses are spherical, enveloped RNA viruses with two-layered icosahedral architecture. The structures of many alphaviruses have been studied using cryogenic electron microscopy (cryo-EM) reconstructions, which impose icosahedral symmetry on the viral particles. Using cryogenic electron tomography (cryo-ET), we revealed a polarized symmetry defect in the icosahedral lattice of Chikungunya virus (CHIKV) in situ, similar to the late budding particles, suggesting the inherent imperfect symmetry originates from the final pinch-off of assembled virions. We further demonstrated this imperfect symmetry is also present in in vitro purified CHIKV and Mayaro virus, another arthritogenic alphavirus. We employed a subparticle-based single-particle analysis protocol to circumvent the icosahedral imperfection and boosted the resolution of the structure of the CHIKV to ∼3 Å resolution, which revealed detailed molecular interactions between glycoprotein E1-E2 heterodimers in the transmembrane region and multiple lipid-like pocket factors located in a highly conserved hydrophobic pocket. This complementary use of in situ cryo-ET and single-particle cryo-EM approaches provides a more precise structural description of near-icosahedral viruses and valuable insights to guide the development of structure-based antiviral therapies against alphaviruses.

PubMed: 38525304
DOI: 10.1093/pnasnexus/pgae102

実験手法

ELECTRON MICROSCOPY (3.09 Å)