8F73

Crystal structure of Pseudomonas aeruginosa UDP-glucose phosphorylase in complex with UDP-glucose

8F73 の概要

• エントリーDOI: 10.2210/pdb8f73/pdb
• 分子名称: UTP--glucose-1-phosphate uridylyltransferase, URIDINE-5'-DIPHOSPHATE-GLUCOSE, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: ugp, udp-glucose pyrophosphorylase, utp:glucose-1-phosphate uridylyltransferase, transferase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 259567.54

構造登録者

Dirr, L.,Fuehring, J. (登録日: 2022-11-17, 公開日: 2023-11-29, 最終更新日: 2024-11-06)

主引用文献

Dirr, L.,Cleeves, S.,Ramon Roth, I.,Li, L.,Fiebig, T.,Ve, T.,Haussler, S.,Braun, A.,von Itzstein, M.,Fuhring, J.I.
Tetramerization is essential for the enzymatic function of the Pseudomonas aeruginosa virulence factor UDP-glucose pyrophosphorylase.
Mbio, 15:e0211423-e0211423, 2024

PubMed Abstract: Multidrug-resistant bacteria such as the opportunistic pathogen , which causes life-threatening infections especially in immunocompromised individuals and cystic fibrosis patients, pose an increasing threat to public health. In the search for new treatment options, uridine diphosphate-glucose pyrophosphorylase (PaUGP) has been proposed as a novel drug target because it is required for the biosynthesis of important virulence factors and linked to pathogenicity in animal models. Here, we show that UGP-deficient exhibits severely reduced virulence against human lung tissue and cells, emphasizing the enzyme's suitability as a drug target. To establish a basis for the development of selective PaUGP inhibitors, we solved the product-bound crystal structure of tetrameric PaUGP and conducted a comprehensive structure-function analysis, identifying key residues at two different molecular interfaces that are essential for tetramer integrity and catalytic activity and demonstrating that tetramerization is pivotal for PaUGP function. Importantly, we show that part of the PaUGP oligomerization interface is uniquely conserved across bacterial UGPs but does not exist in the human enzyme, therefore representing an allosteric site that may be targeted to selectively inhibit bacterial UGPs.IMPORTANCEInfections with the opportunistic bacterial pathogen are becoming increasingly difficult to treat due to multidrug resistance. Here, we show that the enzyme uridine diphosphate-glucose pyrophosphorylase (UGP) is involved in virulence toward human lung tissue and cells, making it a potential target for the development of new antibacterial drugs. Our exploration of (Pa)UGP structure-function relationships reveals that the activity of PaUGP depends on the formation of a tetrameric enzyme complex. We found that a molecular interface involved in tetramer formation is conserved in all bacterial UGPs but not in the human enzyme, and therefore hypothesize that it provides an ideal point of attack to selectively inhibit bacterial UGPs and exploit them as drug targets.

PubMed: 38470050
DOI: 10.1128/mbio.02114-23

実験手法

X-RAY DIFFRACTION (2.9 Å)