8F6D

Crystal structure of the CNNM2 CBS-pair domain in complex with ARL15

8F6D の概要

• エントリーDOI: 10.2210/pdb8f6d/pdb
• 分子名称: Metal transporter CNNM2, ADP-ribosylation factor-like protein 15 (2 entities in total)
• 機能のキーワード: protein complex, cation transport, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 149281.53

構造登録者

Kozlov, G.,Mahbub, L.,Gehring, K. (登録日: 2022-11-16, 公開日: 2023-07-12, 最終更新日: 2024-01-24)

主引用文献

Mahbub, L.,Kozlov, G.,Zong, P.,Lee, E.L.,Tetteh, S.,Nethramangalath, T.,Knorn, C.,Jiang, J.,Shahsavan, A.,Yue, L.,Runnels, L.,Gehring, K.
Structural insights into regulation of CNNM-TRPM7 divalent cation uptake by the small GTPase ARL15.
Elife, 12:-, 2023

PubMed Abstract: Cystathionine-β-synthase (CBS)-pair domain divalent metal cation transport mediators (CNNMs) are an evolutionarily conserved family of magnesium transporters. They promote efflux of Mg ions on their own and influx of divalent cations when expressed with the transient receptor potential ion channel subfamily M member 7 (TRPM7). Recently, ADP-ribosylation factor-like GTPase 15 (ARL15) has been identified as CNNM-binding partner and an inhibitor of divalent cation influx by TRPM7. Here, we characterize ARL15 as a GTP and CNNM-binding protein and demonstrate that ARL15 also inhibits CNNM2 Mg efflux. The crystal structure of a complex between ARL15 and CNNM2 CBS-pair domain reveals the molecular basis for binding and allowed the identification of mutations that specifically block binding. A binding deficient ARL15 mutant, R95A, failed to inhibit CNNM and TRPM7 transport of Mg and Zn ions. Structural analysis and binding experiments with phosphatase of regenerating liver 2 (PRL2 or PTP4A2) showed that ARL15 and PRLs compete for binding CNNM to coordinate regulation of ion transport by CNNM and TRPM7.

PubMed: 37449820
DOI: 10.7554/eLife.86129

実験手法

X-RAY DIFFRACTION (3.2 Å)