8F69

Crystal structure of murine PolG2 dimer bound to DNA

8F69 の概要

• エントリーDOI: 10.2210/pdb8f69/pdb
• 分子名称: DNA polymerase subunit gamma-2, mitochondrial, DNA (5'-D(*CP*TP*GP*GP*TP*AP*GP*GP*CP*GP*CP*CP*TP*AP*CP*CP*AP*G)-3'), 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: dna complex, polg accessory subunit, mitochondria, mtdna, processivity factor, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108795.50

構造登録者

Wojtaszek, J.L.,Hoff, K.E.,Williams, R.S. (登録日: 2022-11-16, 公開日: 2023-08-09, 最終更新日: 2026-03-04)

主引用文献

Wojtaszek, J.L.,Hoff, K.E.,Longley, M.J.,Kaur, P.,Andres, S.N.,Wang, H.,Williams, R.S.,Copeland, W.C.
Structure-specific roles for PolG2-DNA complexes in maintenance and replication of mitochondrial DNA.
Nucleic Acids Res., 51:9716-9732, 2023

PubMed Abstract: The homodimeric PolG2 accessory subunit of the mitochondrial DNA polymerase gamma (Pol γ) enhances DNA binding and processive DNA synthesis by the PolG catalytic subunit. PolG2 also directly binds DNA, although the underlying molecular basis and functional significance are unknown. Here, data from Atomic Force Microscopy (AFM) and X-ray structures of PolG2-DNA complexes define dimeric and hexameric PolG2 DNA binding modes. Targeted disruption of PolG2 DNA-binding interfaces impairs processive DNA synthesis without diminishing Pol γ subunit affinities. In addition, a structure-specific DNA-binding role for PolG2 oligomers is supported by X-ray structures and AFM showing that oligomeric PolG2 localizes to DNA crossings and targets forked DNA structures resembling the mitochondrial D-loop. Overall, data indicate that PolG2 DNA binding has both PolG-dependent and -independent functions in mitochondrial DNA replication and maintenance, which provide new insight into molecular defects associated with PolG2 disruption in mitochondrial disease.

PubMed: 37592734
DOI: 10.1093/nar/gkad679

実験手法

X-RAY DIFFRACTION (2.2 Å)