8F35

Apo ELIC in spMSP1D1 nanodiscs with 2:1:1 POPC:POPE:POPG

8F35 の概要

• エントリーDOI: 10.2210/pdb8f35/pdb
• EMDBエントリー: 28829 28830 28831 28832 41672 41673
• 分子名称: Erwinia chrysanthemi ligand-gated ion channel (1 entity in total)
• 機能のキーワード: elic, ion channel, plgic, structural protein, membrane protein, transport protein
• 由来する生物種: Dickeya dadantii
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 184395.00

構造登録者

Dalal, V.,Arcario, M.J.,Petroff II, J.T.,Deitzen, N.M.,Tan, B.K.,Brannigan, G.,Cheng, W.W.L. (登録日: 2022-11-09, 公開日: 2023-11-15, 最終更新日: 2024-03-27)

主引用文献

Dalal, V.,Arcario, M.J.,Petroff 2nd, J.T.,Tan, B.K.,Dietzen, N.M.,Rau, M.J.,Fitzpatrick, J.A.J.,Brannigan, G.,Cheng, W.W.L.
Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel.
Nat Commun, 15:25-25, 2024

PubMed Abstract: Lipid nanodiscs have become a standard tool for studying membrane proteins, including using single particle cryo-electron microscopy (cryo-EM). We find that reconstituting the pentameric ligand-gated ion channel (pLGIC), Erwinia ligand-gated ion channel (ELIC), in different nanodiscs produces distinct structures by cryo-EM. The effect of the nanodisc on ELIC structure extends to the extracellular domain and agonist binding site. Additionally, molecular dynamic simulations indicate that nanodiscs of different size impact ELIC structure and that the nanodisc scaffold directly interacts with ELIC. These findings suggest that the nanodisc plays a crucial role in determining the structure of pLGICs, and that reconstitution of ion channels in larger nanodiscs may better approximate a lipid membrane environment.

PubMed: 38167383
DOI: 10.1038/s41467-023-44366-w

実験手法

ELECTRON MICROSCOPY (3.17 Å)