8F33

ELIC with Propylamine in saposin nanodiscs with 2:1:1 POPC:POPE:POPG

Summary for 8F33

• Entry DOI: 10.2210/pdb8f33/pdb
• EMDB information: 28829 28830 28831 28832 41672 41673
• Descriptor: Erwinia chrysanthemi ligand-gated ion channel, 3-AMINOPROPANE (2 entities in total)
• Functional Keywords: elic, ion channel, plgic, structural protein, membrane protein, transport protein
• Biological source: Dickeya dadantii
• Total number of polymer chains: 5
• Total formula weight: 184690.55

Authors

Dalal, V.,Arcario, M.J.,Petroff II, J.T.,Deitzen, N.M.,Tan, B.K.,Brannigan, G.,Cheng, W.W.L. (deposition date: 2022-11-09, release date: 2023-11-15, Last modification date: 2024-03-27)

Primary citation

Dalal, V.,Arcario, M.J.,Petroff 2nd, J.T.,Tan, B.K.,Dietzen, N.M.,Rau, M.J.,Fitzpatrick, J.A.J.,Brannigan, G.,Cheng, W.W.L.
Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel.
Nat Commun, 15:25-25, 2024

PubMed Abstract: Lipid nanodiscs have become a standard tool for studying membrane proteins, including using single particle cryo-electron microscopy (cryo-EM). We find that reconstituting the pentameric ligand-gated ion channel (pLGIC), Erwinia ligand-gated ion channel (ELIC), in different nanodiscs produces distinct structures by cryo-EM. The effect of the nanodisc on ELIC structure extends to the extracellular domain and agonist binding site. Additionally, molecular dynamic simulations indicate that nanodiscs of different size impact ELIC structure and that the nanodisc scaffold directly interacts with ELIC. These findings suggest that the nanodisc plays a crucial role in determining the structure of pLGICs, and that reconstitution of ion channels in larger nanodiscs may better approximate a lipid membrane environment.

PubMed: 38167383
DOI: 10.1038/s41467-023-44366-w

Experimental method

ELECTRON MICROSCOPY (3.28 Å)