8F24
Mirror-image RNA octamer containing 2'-OMe-L-uridine
Summary for 8F24
| Entry DOI | 10.2210/pdb8f24/pdb |
| Descriptor | Mirror-image RNA 0G-XEC-0G-0U-0A-0C-0A-0C, SULFATE ION (3 entities in total) |
| Functional Keywords | mirror-image rna, rna |
| Biological source | synthetic construct |
| Total number of polymer chains | 6 |
| Total formula weight | 15723.84 |
| Authors | Dantsu, Y.,Zhang, W. (deposition date: 2022-11-07, release date: 2023-04-12, Last modification date: 2024-04-17) |
| Primary citation | Dantsu, Y.,Zhang, W. Derivatization of Mirror-Image l-Nucleic Acids with 2'-OMe Modification for Thermal and Structural Stabilization. Chembiochem, 24:e202200764-e202200764, 2023 Cited by PubMed Abstract: To further expand the functionality and enhance the stability of mirror-image nucleic acids as advanced agents for basic research and therapeutic design, we have synthesized 2'-deoxy-2'-methoxy-l-uridine phosphoramidite and incorporated it into l-DNA and l-RNA by solid-phase synthesis quantitatively. We found that the thermostability of l-nucleic acids is dramatically improved after introducing the modifications. Moreover, we successfully crystallized both l-DNA and l-RNA duplexes containing the 2'-OMe modifications and sharing identical sequences. Crystal structure determination and analysis revealed the overall structures of the mirror-image nucleic acids, and for the first time it was possible to interpret the structural deviations caused by 2'-OMe and 2'-OH groups in the oligonucleotides, which are very similar. This novel chemical nucleic acid modification has the potential to be used to design nucleic acid-based therapeutics and materials in the future. PubMed: 36892526DOI: 10.1002/cbic.202200764 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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