8EZT

Crystal structure of HipB(Lp) from Legionella pneumophila

8EZT の概要

• エントリーDOI: 10.2210/pdb8ezt/pdb
• 分子名称: HipB(Lp), CHLORIDE ION (3 entities in total)
• 機能のキーワード: toxin-antitoxin complex, legionella pneumophila, structural genomics, center for structural genomics of infectious diseases, csgid, national institute of allergy and infectious diseases, niaid, dna binding protein
• 由来する生物種: Legionella pneumophila
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34708.28

構造登録者

Stogios, P.J.,Skarina, T.,Di Leo, R.,Lin, J.,Ensminger, A.,Savchenko, A.,Joachimiak, A.,Satchell, K.J.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2022-11-01, 公開日: 2023-09-27, 最終更新日: 2026-03-04)

主引用文献

Lin, J.D.,Stogios, P.J.,Abe, K.T.,Wang, A.,MacPherson, J.,Skarina, T.,Gingras, A.-.C.,Savchenko, A.,Ensminger, A.W.
Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila.
Mbio, 14:e0151023-e0151023, 2023

PubMed Abstract: Toxin-antitoxin (TA) systems are parasitic genetic elements found in almost all bacterial genomes. They are exchanged horizontally between cells and are typically poorly conserved across closely related strains and species. Here, we report the characterization of a tripartite TA system in the bacterial pathogen that is highly conserved across species genomes. This system (denoted HipBST) is a distant homolog of the recently discovered split-HipA system in (HipBST). We present bioinformatic, molecular, and structural analyses of the divergence between these two systems and the functionality of this newly described TA system family. Furthermore, we provide evidence to refute previous claims that the toxin in this system (HipT) possesses bifunctionality as an virulence protein. Overall, this work expands our understanding of the split-HipA system architecture and illustrates the potential for undiscovered biology in these abundant genetic elements.

PubMed: 37819088
DOI: 10.1128/mbio.01510-23

実験手法

X-RAY DIFFRACTION (2.06 Å)